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Harvard Bioscience 1% sterile-filtered evans blue dye solution
Comparison of the effect of IP polyP on perfusion and fibrin deposition in the lungs of wild-type (WT) or HRG-deficient mice. (A) <t>Evans</t> <t>blue</t> <t>dye</t> was injected into the right ventricle of WT or HRG−/− mice administered IP saline or polyP. Images of the lungs were taken to examine the extent of dye perfusion into the lungs. Representative images from 6 mice per group are shown. (B) Evans blue dye extracted from lungs with formamide was quantified by measuring absorbance at 605 nm and calculating the amount of dye per microgram of tissue by comparison with a standard curve. N = 6 mice per group; bars represent mean ± standard deviation. (C) Fibrin deposition (red) in lung sections was detected by immunofluorescence with 4′,6-diamidino-2-phenylindole (blue) serving as a nuclear stain. Scale bars represent 50 μm. Representative images from six mice per treatment group are shown. (D) Fibrin intensity was scored in a blinded manner by using a scale of 0 to 4, with 4 representing the greatest intensity. N = 6 mice per group; bars represent mean ± standard deviation. *P < .05, **P < .01, ***P < .001 comparison between saline- or polyP-treated WT or HRG−/− mice as indicated by the lines (analysis of variance, Holm-Šídák method).
1% Sterile Filtered Evans Blue Dye Solution, supplied by Harvard Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Promega evans blue (eb) promega, beijing, china
Comparison of the effect of IP polyP on perfusion and fibrin deposition in the lungs of wild-type (WT) or HRG-deficient mice. (A) <t>Evans</t> <t>blue</t> <t>dye</t> was injected into the right ventricle of WT or HRG−/− mice administered IP saline or polyP. Images of the lungs were taken to examine the extent of dye perfusion into the lungs. Representative images from 6 mice per group are shown. (B) Evans blue dye extracted from lungs with formamide was quantified by measuring absorbance at 605 nm and calculating the amount of dye per microgram of tissue by comparison with a standard curve. N = 6 mice per group; bars represent mean ± standard deviation. (C) Fibrin deposition (red) in lung sections was detected by immunofluorescence with 4′,6-diamidino-2-phenylindole (blue) serving as a nuclear stain. Scale bars represent 50 μm. Representative images from six mice per treatment group are shown. (D) Fibrin intensity was scored in a blinded manner by using a scale of 0 to 4, with 4 representing the greatest intensity. N = 6 mice per group; bars represent mean ± standard deviation. *P < .05, **P < .01, ***P < .001 comparison between saline- or polyP-treated WT or HRG−/− mice as indicated by the lines (analysis of variance, Holm-Šídák method).
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Beijing Solarbio Science evan's blue stain solution
Comparison of the effect of IP polyP on perfusion and fibrin deposition in the lungs of wild-type (WT) or HRG-deficient mice. (A) <t>Evans</t> <t>blue</t> <t>dye</t> was injected into the right ventricle of WT or HRG−/− mice administered IP saline or polyP. Images of the lungs were taken to examine the extent of dye perfusion into the lungs. Representative images from 6 mice per group are shown. (B) Evans blue dye extracted from lungs with formamide was quantified by measuring absorbance at 605 nm and calculating the amount of dye per microgram of tissue by comparison with a standard curve. N = 6 mice per group; bars represent mean ± standard deviation. (C) Fibrin deposition (red) in lung sections was detected by immunofluorescence with 4′,6-diamidino-2-phenylindole (blue) serving as a nuclear stain. Scale bars represent 50 μm. Representative images from six mice per treatment group are shown. (D) Fibrin intensity was scored in a blinded manner by using a scale of 0 to 4, with 4 representing the greatest intensity. N = 6 mice per group; bars represent mean ± standard deviation. *P < .05, **P < .01, ***P < .001 comparison between saline- or polyP-treated WT or HRG−/− mice as indicated by the lines (analysis of variance, Holm-Šídák method).
Evan's Blue Stain Solution, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Macklin Inc evans blue eb cas:314-13-6 cas:314-13-6
Comparison of the effect of IP polyP on perfusion and fibrin deposition in the lungs of wild-type (WT) or HRG-deficient mice. (A) <t>Evans</t> <t>blue</t> <t>dye</t> was injected into the right ventricle of WT or HRG−/− mice administered IP saline or polyP. Images of the lungs were taken to examine the extent of dye perfusion into the lungs. Representative images from 6 mice per group are shown. (B) Evans blue dye extracted from lungs with formamide was quantified by measuring absorbance at 605 nm and calculating the amount of dye per microgram of tissue by comparison with a standard curve. N = 6 mice per group; bars represent mean ± standard deviation. (C) Fibrin deposition (red) in lung sections was detected by immunofluorescence with 4′,6-diamidino-2-phenylindole (blue) serving as a nuclear stain. Scale bars represent 50 μm. Representative images from six mice per treatment group are shown. (D) Fibrin intensity was scored in a blinded manner by using a scale of 0 to 4, with 4 representing the greatest intensity. N = 6 mice per group; bars represent mean ± standard deviation. *P < .05, **P < .01, ***P < .001 comparison between saline- or polyP-treated WT or HRG−/− mice as indicated by the lines (analysis of variance, Holm-Šídák method).
Evans Blue Eb Cas:314 13 6 Cas:314 13 6, supplied by Macklin Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Nacalai physiological saline solution containing 1% evans blue dye nacalai tesque, cat# 09158-74
Comparison of the effect of IP polyP on perfusion and fibrin deposition in the lungs of wild-type (WT) or HRG-deficient mice. (A) <t>Evans</t> <t>blue</t> <t>dye</t> was injected into the right ventricle of WT or HRG−/− mice administered IP saline or polyP. Images of the lungs were taken to examine the extent of dye perfusion into the lungs. Representative images from 6 mice per group are shown. (B) Evans blue dye extracted from lungs with formamide was quantified by measuring absorbance at 605 nm and calculating the amount of dye per microgram of tissue by comparison with a standard curve. N = 6 mice per group; bars represent mean ± standard deviation. (C) Fibrin deposition (red) in lung sections was detected by immunofluorescence with 4′,6-diamidino-2-phenylindole (blue) serving as a nuclear stain. Scale bars represent 50 μm. Representative images from six mice per treatment group are shown. (D) Fibrin intensity was scored in a blinded manner by using a scale of 0 to 4, with 4 representing the greatest intensity. N = 6 mice per group; bars represent mean ± standard deviation. *P < .05, **P < .01, ***P < .001 comparison between saline- or polyP-treated WT or HRG−/− mice as indicated by the lines (analysis of variance, Holm-Šídák method).
Physiological Saline Solution Containing 1% Evans Blue Dye Nacalai Tesque, Cat# 09158 74, supplied by Nacalai, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Fluka Chemical bisazo dye evans blue (eb, fluka co)
Comparison of the effect of IP polyP on perfusion and fibrin deposition in the lungs of wild-type (WT) or HRG-deficient mice. (A) <t>Evans</t> <t>blue</t> <t>dye</t> was injected into the right ventricle of WT or HRG−/− mice administered IP saline or polyP. Images of the lungs were taken to examine the extent of dye perfusion into the lungs. Representative images from 6 mice per group are shown. (B) Evans blue dye extracted from lungs with formamide was quantified by measuring absorbance at 605 nm and calculating the amount of dye per microgram of tissue by comparison with a standard curve. N = 6 mice per group; bars represent mean ± standard deviation. (C) Fibrin deposition (red) in lung sections was detected by immunofluorescence with 4′,6-diamidino-2-phenylindole (blue) serving as a nuclear stain. Scale bars represent 50 μm. Representative images from six mice per treatment group are shown. (D) Fibrin intensity was scored in a blinded manner by using a scale of 0 to 4, with 4 representing the greatest intensity. N = 6 mice per group; bars represent mean ± standard deviation. *P < .05, **P < .01, ***P < .001 comparison between saline- or polyP-treated WT or HRG−/− mice as indicated by the lines (analysis of variance, Holm-Šídák method).
Bisazo Dye Evans Blue (Eb, Fluka Co), supplied by Fluka Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Comparison of the effect of IP polyP on perfusion and fibrin deposition in the lungs of wild-type (WT) or HRG-deficient mice. (A) Evans blue dye was injected into the right ventricle of WT or HRG−/− mice administered IP saline or polyP. Images of the lungs were taken to examine the extent of dye perfusion into the lungs. Representative images from 6 mice per group are shown. (B) Evans blue dye extracted from lungs with formamide was quantified by measuring absorbance at 605 nm and calculating the amount of dye per microgram of tissue by comparison with a standard curve. N = 6 mice per group; bars represent mean ± standard deviation. (C) Fibrin deposition (red) in lung sections was detected by immunofluorescence with 4′,6-diamidino-2-phenylindole (blue) serving as a nuclear stain. Scale bars represent 50 μm. Representative images from six mice per treatment group are shown. (D) Fibrin intensity was scored in a blinded manner by using a scale of 0 to 4, with 4 representing the greatest intensity. N = 6 mice per group; bars represent mean ± standard deviation. *P < .05, **P < .01, ***P < .001 comparison between saline- or polyP-treated WT or HRG−/− mice as indicated by the lines (analysis of variance, Holm-Šídák method).

Journal: Blood Advances

Article Title: Polyphosphate-induced thrombosis in mice is factor XII dependent and is attenuated by histidine-rich glycoprotein

doi: 10.1182/bloodadvances.2021004567

Figure Lengend Snippet: Comparison of the effect of IP polyP on perfusion and fibrin deposition in the lungs of wild-type (WT) or HRG-deficient mice. (A) Evans blue dye was injected into the right ventricle of WT or HRG−/− mice administered IP saline or polyP. Images of the lungs were taken to examine the extent of dye perfusion into the lungs. Representative images from 6 mice per group are shown. (B) Evans blue dye extracted from lungs with formamide was quantified by measuring absorbance at 605 nm and calculating the amount of dye per microgram of tissue by comparison with a standard curve. N = 6 mice per group; bars represent mean ± standard deviation. (C) Fibrin deposition (red) in lung sections was detected by immunofluorescence with 4′,6-diamidino-2-phenylindole (blue) serving as a nuclear stain. Scale bars represent 50 μm. Representative images from six mice per treatment group are shown. (D) Fibrin intensity was scored in a blinded manner by using a scale of 0 to 4, with 4 representing the greatest intensity. N = 6 mice per group; bars represent mean ± standard deviation. *P < .05, **P < .01, ***P < .001 comparison between saline- or polyP-treated WT or HRG−/− mice as indicated by the lines (analysis of variance, Holm-Šídák method).

Article Snippet: Briefly, 200 μL of 1% sterile-filtered Evans blue dye solution was injected into the right ventricle over 30 seconds using a syringe pump (Harvard Apparatus, Holliston, MA).

Techniques: Injection, Standard Deviation, Immunofluorescence, Staining